Gene description for FLOT1
Gene name	flotillin 1
Gene symbol	FLOT1
Other names/aliases	-
Species	Homo sapiens
Experiment description of studies that identified FLOT1 in Embryonic kidney cells
1 Experiment ID 925 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry Western blotting PubMed ID 25483805    Organism Homo sapiens Experiment description Vertebrate Hedgehog is secreted on two types of extracellular vesicles with different signaling properties Authors Vyas N, Walvekar A, Tate D, Lakshmanan V, Bansal D, Lo Cicero A, Raposo G, Palakodeti D, Dhawan J. Journal name Sci Rep Publication year 2014 Sample Embryonic kidney cells Sample name HEK293T - P150 - Fraction 1.12 g/mL Isolation/purification methods Differential centrifugation Ultracentrifugation Density gradient centrifugation Flotation density 1.09-1.12 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting EV-TRACK EV140018: EV-METRIC:56%, 56%
2 Experiment ID 787 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 29953723    Organism Homo sapiens Experiment description Stimulation of exosome release by extracellular DNA is conserved across multiple cell types Authors Iliev D, Strandskog G, Nepal A, Aspar A, Olsen R, Jøsen J, Wolfson D, Ahluwalia BS, Handzhiyski J, Mironova R. Journal name FEBS J Publication year 2018 Sample Embryonic kidney cells Sample name HEK293T Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
3 Experiment ID 788 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 29953723    Organism Homo sapiens Experiment description Stimulation of exosome release by extracellular DNA is conserved across multiple cell types Authors Iliev D, Strandskog G, Nepal A, Aspar A, Olsen R, Jøsen J, Wolfson D, Ahluwalia BS, Handzhiyski J, Mironova R. Journal name FEBS J Publication year 2018 Sample Embryonic kidney cells Sample name HEK293T - Fraction 4 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Density gradient centrifugation Flotation density 1.07-1.13 g/mL Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
4 Experiment ID 789 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 29953723    Organism Homo sapiens Experiment description Stimulation of exosome release by extracellular DNA is conserved across multiple cell types Authors Iliev D, Strandskog G, Nepal A, Aspar A, Olsen R, Jøsen J, Wolfson D, Ahluwalia BS, Handzhiyski J, Mironova R. Journal name FEBS J Publication year 2018 Sample Embryonic kidney cells Sample name HEK293T - Fraction 5 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Density gradient centrifugation Flotation density 1.07-1.13 g/mL Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
5 Experiment ID 790 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 29953723    Organism Homo sapiens Experiment description Stimulation of exosome release by extracellular DNA is conserved across multiple cell types Authors Iliev D, Strandskog G, Nepal A, Aspar A, Olsen R, Jøsen J, Wolfson D, Ahluwalia BS, Handzhiyski J, Mironova R. Journal name FEBS J Publication year 2018 Sample Embryonic kidney cells Sample name HEK293T - S. salar genomic DNA stimulated Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
6 Experiment ID 791 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 29953723    Organism Homo sapiens Experiment description Stimulation of exosome release by extracellular DNA is conserved across multiple cell types Authors Iliev D, Strandskog G, Nepal A, Aspar A, Olsen R, Jøsen J, Wolfson D, Ahluwalia BS, Handzhiyski J, Mironova R. Journal name FEBS J Publication year 2018 Sample Embryonic kidney cells Sample name HEK293T - E. coli genomic DNA stimulated Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
7 Experiment ID 792 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 29953723    Organism Homo sapiens Experiment description Stimulation of exosome release by extracellular DNA is conserved across multiple cell types Authors Iliev D, Strandskog G, Nepal A, Aspar A, Olsen R, Jøsen J, Wolfson D, Ahluwalia BS, Handzhiyski J, Mironova R. Journal name FEBS J Publication year 2018 Sample Embryonic kidney cells Sample name HEK293T - condition medium collected after 5 min with S. salar genomic DNA stimulation Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
8 Experiment ID 793 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 29953723    Organism Homo sapiens Experiment description Stimulation of exosome release by extracellular DNA is conserved across multiple cell types Authors Iliev D, Strandskog G, Nepal A, Aspar A, Olsen R, Jøsen J, Wolfson D, Ahluwalia BS, Handzhiyski J, Mironova R. Journal name FEBS J Publication year 2018 Sample Embryonic kidney cells Sample name HEK293T - condition medium collected after 45 min without S.salar genomic DNA stimulation Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
9 Experiment ID 794 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 29953723    Organism Homo sapiens Experiment description Stimulation of exosome release by extracellular DNA is conserved across multiple cell types Authors Iliev D, Strandskog G, Nepal A, Aspar A, Olsen R, Jøsen J, Wolfson D, Ahluwalia BS, Handzhiyski J, Mironova R. Journal name FEBS J Publication year 2018 Sample Embryonic kidney cells Sample name HEK293T - condition medium collected after 45 min with S. salar genomic DNA stimulation Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
10 Experiment ID 797 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 29953723    Organism Homo sapiens Experiment description Stimulation of exosome release by extracellular DNA is conserved across multiple cell types Authors Iliev D, Strandskog G, Nepal A, Aspar A, Olsen R, Jøsen J, Wolfson D, Ahluwalia BS, Handzhiyski J, Mironova R. Journal name FEBS J Publication year 2018 Sample Embryonic kidney cells Sample name HEK293T - DNase I treated Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
11 Experiment ID 798 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 29953723    Organism Homo sapiens Experiment description Stimulation of exosome release by extracellular DNA is conserved across multiple cell types Authors Iliev D, Strandskog G, Nepal A, Aspar A, Olsen R, Jøsen J, Wolfson D, Ahluwalia BS, Handzhiyski J, Mironova R. Journal name FEBS J Publication year 2018 Sample Embryonic kidney cells Sample name HEK293T - DNase I treated with S. salar genomic DNA stimulation Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
12 Experiment ID 922 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 25483805    Organism Homo sapiens Experiment description Vertebrate Hedgehog is secreted on two types of extracellular vesicles with different signaling properties Authors Vyas N, Walvekar A, Tate D, Lakshmanan V, Bansal D, Lo Cicero A, Raposo G, Palakodeti D, Dhawan J. Journal name Sci Rep Publication year 2014 Sample Embryonic kidney cells Sample name HEK293T - P150 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Immunofluorescence EV-TRACK EV140018: EV-METRIC:56%, 56%
13 Experiment ID 924 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 25483805    Organism Homo sapiens Experiment description Vertebrate Hedgehog is secreted on two types of extracellular vesicles with different signaling properties Authors Vyas N, Walvekar A, Tate D, Lakshmanan V, Bansal D, Lo Cicero A, Raposo G, Palakodeti D, Dhawan J. Journal name Sci Rep Publication year 2014 Sample Embryonic kidney cells Sample name HEK293T - P150 - Fraction 1.09 g/mL Isolation/purification methods Differential centrifugation Ultracentrifugation Density gradient centrifugation Flotation density 1.09-1.12 g/mL Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK EV140018: EV-METRIC:56%, 56%
14 Experiment ID 1671 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 34108659    Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T - Exo-rich fractions 7-10 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry EV-TRACK -
15 Experiment ID 1673 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 34108659    Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T - Fraction 1 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
16 Experiment ID 1674 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 34108659    Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T - Fraction 2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
17 Experiment ID 1675 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 34108659    Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T - Fraction 3 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
18 Experiment ID 1676 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 34108659    Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T - Fraction 4 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
19 Experiment ID 1677 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 34108659    Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T - Fraction 5 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
20 Experiment ID 1408 Identified molecule Protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Mass spectrometry PubMed ID 36706192    Organism Homo sapiens Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles Authors Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q. Journal name Sci Adv Publication year 2023 Sample Embryonic kidney cells Sample name HEK293T Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry EV-TRACK -
21 Experiment ID 1413 Identified molecule Protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Mass spectrometry PubMed ID 36706192    Organism Homo sapiens Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles Authors Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q. Journal name Sci Adv Publication year 2023 Sample Embryonic kidney cells Sample name HEK293T - Fraction 5 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density 1.151 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry EV-TRACK -
22 Experiment ID 1659 Identified molecule Protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Mass spectrometry PubMed ID 34108659    Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein mRNA Methods used in the study RT-qPCR Western blotting Mass spectrometry Flow cytometry EV-TRACK -
23 Experiment ID 1671 Identified molecule Protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Mass spectrometry PubMed ID 34108659    Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T - Exo-rich fractions 7-10 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry EV-TRACK -
24 Experiment ID 1671 Identified molecule Protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Mass spectrometry PubMed ID 34108659    Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T - Exo-rich fractions 7-10 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry EV-TRACK -
25 Experiment ID 1672 Identified molecule Protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Mass spectrometry PubMed ID 34108659    Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T - Exo-rich fractions 1-6 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK -
26 Experiment ID 1672 Identified molecule Protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Mass spectrometry PubMed ID 34108659    Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T - Exo-rich fractions 1-6 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK -